TM
VirionBrain
Gene therapy testing
Virus invasion model
Human brain-virus organoid model
Advantages of VirionBrain
Gene therapy testing
Virus invasion model
Short & Long term compound testing
Human 3-dimension tissues
Highly physiological virus - brain organoid interactions
Monitoring viral spreading and affected cell populations
TM
VirionBrain is a service for testing the efficiency of gene therapy or anti-viral compounds. We generate 3-dimensional neural tissue derived from human pluripotent stem cells, these are inoculated with virus.
The long term compound testing, 3D human organoids and physiological neural-virus interactions makes VirionBrain advantageous compared to currently used 2D cellular and animal models.
VirionBrain represents an ideal tool for gene therapy or anti-viral compound screening and validation through efficiency testing, or deeper histological, proteomic and genomic testing for mechanistic studies.
Virus spread among neural tissue
Cytomegalovirus, Enterovirus and Zika virus showed potent invasion in our human neural tissue.
Fig. A: Left panel shows progression of the red-stained cytomegalovirus (CMV) in the 3D neural tissue. Right panel display virus copy number following infection of the organoid by CMV (black), Enterovirus D94 (grey) and African Zika (white).
Monitoring affected cell populations
Each virus may infect different cell types. Histological analysis can be performed and shows that CMV specifically affects neural progenitors and weakly neurons.
Fig. B: Histological analysis neural tissue infected with CMV. human CMV-infected cells (HCMV), neural progenitors (nestin) and neurons (bIII-tub).
Quantification of infected cell types
CMV infection pattern remains the same between a brain from human fetus and our 3D neural tissue.
Fig. C: Cells infected by CMV in a human fetal brain (black) and 3D neural tissue (grey) were compared. CMV infects in equal proportion neural progenitors (nestin marker) and neurons (bIII-tubulin marker).
VirionBrain Virus Invasion Model and Gene Therapy Testing - Service specifications
Cell types
Production technology
Field of application
Assay window
Readouts
TM
3D Neural tissue: Generated from human pluripotent stem cells. Composed of neurons, astrocytes, oligodendrocytes and neural progenitor cells. The latter cells keep generating newborn neurons in a dynamic process.
Neurix’s MinibrainTM & Neurosphere technology with minimum batch to batch variability guaranteed by extensive quality control of identity (rt-qPCR)
High throughput screening and lead treatment validation
Short term (7 days) to long term (one month)
- Virus copy number and/or reporter signal
- 3D reconstruction of infected area and volume quantification
- Quantification of infected cell number
- Analysis of infected cell types
Our publications
Cosset, É. et al. Human three-dimensional engineered neural tissue reveals cellular and molecular events following cytomegalovirus infection. Biomaterials 53, 296–308 (2015).
Royston, L. et al. Viral chimeras decrypt the role of enterovirus capsid proteins in viral tropism, acid sensitivity and optimal growth temperature. PLoS Pathog. 14, e1006962 (2018).
Cagno, V. et al. Growth of Zika virus in human reconstituted respiratory, intestinal, vaginal and neural tissues. Clin. Microbiol. Infect. Off. Publ. Eur. Soc. Clin. Microbiol. Infect. Dis. 25, 1042.e1-1042.e4 (2019).
Validated assay and protocols
VirionBrain service is integrated into a variety of validated assay that can be implemented in drug development for efficacy evaluation of novel compounds:
Cell viability assays
Histological analysis (IHC & IF)
Cell sorting and cell population analysis (FACS)
Genomic analysis
Proteomic analysis
Get in contact with us
Neurix offers customized services for neural applications. These include gene / cell / polymer therapy testing, brain tumor drug testing, neurodegenerative diseases modeling and neurotoxicity assays. Our experienced scientists are happy to work with you in order to understand your needs and meet your objectives.
Email: info@neurix.ch
Phone: +41 22 379 46 43
Location: Avenue de la Roseraie 64
1205 Geneva, Switzerland